Journal: Redox Biology

Article Title: Targeting nitrative stress for attenuating cisplatin-induced downregulation of cochlear LIM domain only 4 and ototoxicity

doi: 10.1016/j.redox.2016.10.016

Figure Lengend Snippet: Cisplatin-induced increase in nitrotyrosine is attenuated by SRI110. A) Increased levels of nitrotyrosine as well as condensed nuclei (white arrows) were detected in UBOC1 cells treated with 10 µm cisplatin. Co-treatment with 50 µm SRI110 attenuated the cisplatin-induced increase in nitrotyrosine. Red staining indicates immunoreactivity to anti-nitrotyrosine, blue indicates nuclear staining with DAPI, while green indicates actin staining with phalloidin. The images are representative of four biological replicates. Scale bar, 20 µm. (B) The mean pixel intensities for nitrotyrosine immunostaining was significantly higher in cisplatin treated cells (****P<0.0001), which was attenuated by SRI110 co-treatment (***P<0.001). The results are expressed as mean±standard deviation, n=4. (C) Immunostaining (violet) with anti-myosin VIIa indicated the differentiated state of UBOC1 cells. The image is representative of three biological replicates. Scale bar, 20 µm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Then the cells were incubated with anti-nitrotyrosine, anti-myosin VIIa (catalog no. sc-32757, sc-74516, Santa Cruz Biotechnology Inc., Santa Cruz, CA) or anti-LMO4 (catalog no. ab39383, Abcam, Cambridge, MA) followed by incubation with Alexa Fluor 568 donkey anti-mouse or Alexa Fluor 647 goat anti-rabbit secondary antibody (catalog no. A10037 or A21244, Life Technologies, Carlsbad, CA) and fluorescein phalloidin (catalog no. F432, Life Technologies).

Techniques: Staining, Immunostaining, Standard Deviation

Journal: PLoS ONE

Article Title: EIAV-Based Retinal Gene Therapy in the shaker1 Mouse Model for Usher Syndrome Type 1B: Development of UshStat

doi: 10.1371/journal.pone.0094272

Figure Lengend Snippet: A . Schematic diagram showing the genetic structure of the integrated EIAV vectors used in this study . EIAV-CMV-MYO7A (UshStat) is based on a non-replicating non-human recombinant lentiviral vector based on the non-pathogenic wild type equine infectious anaemia virus (EIAV). The wild-type EIAV virus has 6 distinct genetic units, however, the majority of these EIAV sequences have been removed to produce a minimal vector system that contains less than 10% of the original viral genome and does not contain any viral promoters or enhancers and there are no coding regions for accessory proteins in either the EIAV genome or in the packaging system. SIN LTR: Self inactivating long term repeat. Neo: Neomycin open reading frame (ORF). CMV: Cytomegalovirus promoter (constitutive). RK: Rhodopsin kinase promoter (photoreceptor specific). eGFP: enhanced green fluorescent protein ORF. MYO7A: Myosin VIIa ORF. WPRE: Woodchuk hepatitis virus post-transcriptional regulatory element. B . Expression analysis of myosin VIIa in 4 weeks mouse eye and HeLa cells transfected with the EIAV-CMV-Null (Null) or UshStat constructs. β-actin was used as loading control. RPE: retinal pigment epithelium. NR: neuroretina. IP/Null: immunoprecipitates of HeLa cells transfected with the null vector. IP/UshStat: immunoprecipitates of HeLa cells transfected with the myosin VIIa vector. IB MYO7A: immunoblot with the mouse anti-myosin VIIa. Molecular weight markers are denoted to the left. C–D : Immunocytochemistry studies of HeLa cells transduced with the null ( C ) or the UshStat vector ( D ) and immunostained for myosin VIIa (red). DAPI was used to counter stain the nucleus. Scale bar: 15 μm.

Article Snippet: For immunoprecipitation studies, 40 μl of protein-A sepharose beads (50% slurry, Sigma, MI) were incubated with 4 μg of rabbit anti-myosin VIIa (Novus Biologicals, CO) overnight at 4°C.

Techniques: Recombinant, Plasmid Preparation, Virus, Expressing, Transfection, Construct, Control, Western Blot, Molecular Weight, Immunocytochemistry, Transduction, Staining

Journal: PLoS ONE

Article Title: EIAV-Based Retinal Gene Therapy in the shaker1 Mouse Model for Usher Syndrome Type 1B: Development of UshStat

doi: 10.1371/journal.pone.0094272

Figure Lengend Snippet: Shaker1 mouse retinas were co-transduced by a subretinal injection of UshStat and EIAV-CMV-GFP or EIAV-CMV-Null and EIAV-RK-GFP (the GFP vector was used to identify the transduced region of the retina). After 4 weeks the animals were dark-adapted overnight, then light-adapted for 10 minutes under 200 lux illumination. Retinas were double immunostained with antibodies against GFP (green) and α-transducin (red). Panel I : Low magnification image of a retina transduce with UshStat and GFP, showing that the gradient in α-transducin translocation (left to right) parallels GFP expression (indicative of wild type myosin VIIa). Scale bar: 40 μm. Panel II : A–C : Representative examples of an EIAV transduced region of the retina (determine by the presence of GFP), presenting dual immunostaining for GFP and α-transducin ( C ). In the presence of wild type myosin VIIa, α-transducin is translocated to the inner segment (IS). D–F : An untransduced region of the same retina as in A–C does not show α-transducin translocation to the IS upon illumination. G–I : Retinas co-transduced with EIAV-Null-vector and EIAV-RK-GFP (photoreceptor cell-specific promoter) as a control for the effects of subretinal lentiviral transduction on α-transducin translocation. The region of the retina shown was transduced, evidenced by GFP expression in the photoreceptors ( H ) however there was no translocation of α-transducin to the IS ( G and I ). The qualitative results represented in each of the panels are representative images for at least three replicate experiments. RPE = Retinal Pigment Epithelium; OS = Outer Segments; IS = Inner Segments; ONL = Outer Nuclear Layer; OPL = Outer Plexiform Layer. Scale bar: 25 μm. Arrowheads in D and G indicate translocation of α-transducin in individual photoreceptors. Asterisks in I denote reactivity of the secondary anti-mouse antibody with circulation mouse IgGs present in the blood vessels.

Article Snippet: For immunoprecipitation studies, 40 μl of protein-A sepharose beads (50% slurry, Sigma, MI) were incubated with 4 μg of rabbit anti-myosin VIIa (Novus Biologicals, CO) overnight at 4°C.

Techniques: Injection, Plasmid Preparation, Translocation Assay, Expressing, Immunostaining, Transduction, Control

Journal: PLoS ONE

Article Title: EIAV-Based Retinal Gene Therapy in the shaker1 Mouse Model for Usher Syndrome Type 1B: Development of UshStat

doi: 10.1371/journal.pone.0094272

Figure Lengend Snippet: A–B : Show myosin VIIa immunostaining of shaker1 retinas transduced with the EIAV-CMV-Null vector ( A ) or UshStat ( B ). Myosin VIIa expression can be detected in the RPE, OS and IS regions. C–D : monkey retinas transduced with UshStat ( D ) showed high levels of human myosin VIIa in the RPE and moderate expression in outer and inner segments of the photoreceptor cells. Asterisk in C denotes weak immunostaining of the endogenous myosin VIIa in the RPE layer. Note that the anti-myosin VIIa antibody was titrated so as to only detect the exogenous overexpressed virus-derived wild type myosin VIIa ( B, D ) and not the endogenous protein ( A, C ). Scale bars: 25 μm.

Article Snippet: For immunoprecipitation studies, 40 μl of protein-A sepharose beads (50% slurry, Sigma, MI) were incubated with 4 μg of rabbit anti-myosin VIIa (Novus Biologicals, CO) overnight at 4°C.

Techniques: Immunostaining, Transduction, Plasmid Preparation, Expressing, Virus, Derivative Assay

Journal: PLoS ONE

Article Title: EIAV-Based Retinal Gene Therapy in the shaker1 Mouse Model for Usher Syndrome Type 1B: Development of UshStat

doi: 10.1371/journal.pone.0094272

Figure Lengend Snippet: Retinas were co-transduced with EIAV-CMV-Null vector and EIAV-RK-GFP ( A–C ) or UshStat and EIAV-CMV-GFP ( D–F, J ). After 4 weeks, mice were exposed to 6 days continuous light at 2000 lux illumination. Retinas were harvested and dual immunostained for myosin VIIa (red) and GFP (green) ( A–F, J ). Immunostaining conditions were chosen as to only detect the exogenous overexpressed myosin VIIa. G–H : Eosin and hematoxylin histochemical staining of shaker1 mouse retinas transduced with either the EIAV-CMV-Null vector ( G ) or UshStat ( H ). I : Eosin and hematoxylin staining of wild type (untransduced) retina for comparison of relative light dependent degeneration of the ONL that is typically observed (double headed arrow). J : Low magnification image of an UshStat+GFP co-transduced retina, showing the area used for the studies (bracket) relative to the area of injection (syringe). This area corresponds to the juxtaposed ∼0.2 mm from the site of injection. K : Schematic representation of the area of injection represented by red boxes, where right box area corresponds to the injection in the right eye and left box area injection in the left eye. The inferior retina was always used for the injections and ONL counting. Scale bars A–F : 25 μm, G–I : 50 μm. J : 90 μm. Labels are as in . Asterisks in C , F and J denote reactivity of the secondary anti-mouse antibody with circulation mouse IgGs present in the blood vessels.

Article Snippet: For immunoprecipitation studies, 40 μl of protein-A sepharose beads (50% slurry, Sigma, MI) were incubated with 4 μg of rabbit anti-myosin VIIa (Novus Biologicals, CO) overnight at 4°C.

Techniques: Transduction, Plasmid Preparation, Immunostaining, Staining, Comparison, Injection

Journal: Aging Cell

Article Title: Morphological phenotyping of the aging cochlea in inbred C57BL/6N and outbred CD1 mouse strains

doi: 10.1111/acel.14362

Figure Lengend Snippet: List of the primary and secondary antibodies used in the study.

Article Snippet: Myosin VIIa , Rabbit , GTX23481 , GeneTex , 1:100.

Techniques:

Journal: European Journal of Histochemistry : EJH

Article Title: Apoptosis pattern and alterations of expression of apoptosis-related factors of supporting cells in Kölliker’s organ in vivo in early stage after birth in rats

doi: 10.4081/ejh.2017.2706

Figure Lengend Snippet: Immunofluorescence staining of basilar membrane of P1 rats. The apical (A), middle (B), and basal (C) turns were displayed. Myosin-VIIa labeled hair cells (green); Sox2 marks all supporting cells, including Kölliker’s supporting cells (red). The number of SCs in Kölliker’s organ decreased with an apex-to-base gradient. Scale bars: 50 µm.

Article Snippet: They were subsequently incubated with goat anti-myosin VIIa (1:300, Proteus BD BioSciences, San Jose, CA, USA) and rabbit anti-Sox2 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies in 5% normal donkey serum and 0.1% Triton X-100 in 0.01 M PBS overnight at 4°C, mediated by DyLight 594 and DyLight 488 conjugated secondary antibodies (goat anti-rabbit IgG or rabbit anti-goat IgG) at a concentration of 1:500 for 1 h at room temperature.

Techniques: Immunofluorescence, Staining, Labeling

Journal: bioRxiv

Article Title: In Silico Transcriptome-based Screens Identify Epidermal Growth Factor Receptor Inhibitors as Therapeutics for Noise-induced Hearing Loss

doi: 10.1101/2023.06.07.544128

Figure Lengend Snippet: Afatinib and zorifertinib protect against noise-induced cochlear synaptopathy in mice. ( A ) Representative maximum intensity projections of inner hair cells (IHCs) in the 16-22 kHz region of the cochlea following drug treatment and with (left) or without noise (right). Hair cells were labeled using myosin-VIIa, presynaptic puncta were labeled using CtBP2, and nuclei were counterstained with Hoechst. ( B ) Zorifertinib and Afatinib protect against noise induced cochlear synaptopathy, resulting in less CtBP2 puncta loss with drug + noise than control + noise. The number of CtBP2 puncta per inner hair cell (IHC) is expressed as mean +/- SD; n=2- 5 animals per group. Each dot (n) represents one animal and the average CtBP2 puncta across ten IHCs from two cochleae. *P<0.05. **P<0.01, ***P<0.001; Welch’s ANOVA. Z: Zorifertinib; A: Afatinib; C: Control; N: Noise.

Article Snippet: Antibodies used included: C terminal binding protein-2 (mouse anti-CtBP2; BD Transduction Labs, used at 1:200), myosin-VIIA (rabbit anti-myosin-VIIA; Proteus Biosciences, used at 1:250), anti-otoferlin (HCS-1, DSHB 1:500), anti-GFP (NB100-1614, Novus Biologicals 1:500), total AKT and ERK (Cell Signaling 9272S and 4695S, respectively), phospho forms (AKT-S473 and ERK1/2-T202/Y204, Cell Signaling) and β-actin (Sigma A3854).

Techniques: Labeling, Control

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Regeneration of Cochlear Hair Cells and Hearing Recovery through Hes1 Modulation with siRNA Nanoparticles in Adult Guinea Pigs.

doi: 10.1016/j.ymthe.2018.03.004

Figure Lengend Snippet: Figure 3. Supernumerary IHCs Were Uniquely Observed in siHes1 NP-Treated Cochleae (A–F)HCs were immunolabeled withanti-myosin VIIa(green in A, B, E, and F), whereas stereocilia were labeled with fluorophore-conjugated phalloidin (yellow in C–F). Nuclei were stained with DAPI (blue in E and F). Supernumerary IHCs (arrows in B and F) were observed only in noise- deafened OCs treated with siHes1 NPs. Some ectopic IHCs possessed phalloidin-labeled stereocilia (arrowheads in D and F), whereas some presented with no stereocilia (arrows in F). No ectopic HCs were observed in cochleae treated with scRNA NPs (A and E). (G and H) Scanning electron microscope image of an ectopic IHC in an siHes1- NP-treated OC is shown. Ectopic IHCs (arrow in G) with stereociliary structures were also observed by scanning electron microscopy in OCs at three weeks after siHes1 NP treatment (image collected from the 2nd turn of the OC). At this time point, the majority of HCs possessed stereocilia with normal morphology (G). I, P, and O in (G) indicate IHCs, pillar cells, and OHCs, respectively. The scale bars repre- sent 50 mm in (F) for (A)–(F), 10 mm in (G), and 1 mm in (H).

Article Snippet: After blocking, the tissues were incubated with rabbit anti-myosin VIIa, goat anti-prestin (1:20; cat no.: OAEB00391; Aviva Systems Biology, San Diego, CA), and mouse anti-vGlut3 (1:25; cat no.: 135211; Synaptic Systems, Goettingen, Germany) at 4 C overnight.

Techniques: Immunolabeling, Labeling, Staining, Microscopy, Electron Microscopy

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Regeneration of Cochlear Hair Cells and Hearing Recovery through Hes1 Modulation with siRNA Nanoparticles in Adult Guinea Pigs.

doi: 10.1016/j.ymthe.2018.03.004

Figure Lengend Snippet: Figure 4. Immature OHCs Were Observed in OCs Treated with siHes1 NPs Immature OHCs were observed in OCs treated with siHes1 NPs by differential prestin immunolabeling at nine (A–H’) and three (I and I’) weeks after treatment. HCs were immunolabeled by anti-myosin VIIa (green in A, B, B’, and G–I’), whereas mature OHCs were immunola- beled with anti-prestin (pink in C–D’ and G–I’). Nuclei were labeled with DAPI (blue in E–I’). Arrowheads in B, D, and H indicate a myosin-VIIa-positive/prestin-negative immature OHC in the 2nd turn of a noise-injured OC treated with siHes1 NPs at nine weeks post- treatment. This immature OHC has an apparently normal OHC morphology (H’). Arrowheads in (I) indicate two myosin-VIIa-positive/prestin-negative OHCs adjacent to a myosin-VIIa-positive/prestin-positive OHC in a noise- injured OC at three weeks post-siHes1 NP treatment. These OHCs were anucleate and small in size (I’). All OHCs in noise-deafened ears treated with scRNA NPs possessed dual labeling with myosin Vlla and prestin (G). The scale bars represent 10 mm in (I) and (I’) and apply to (A)–(I) and (B’), (D’), (F’), (H’), and (I’), respectively.

Article Snippet: After blocking, the tissues were incubated with rabbit anti-myosin VIIa, goat anti-prestin (1:20; cat no.: OAEB00391; Aviva Systems Biology, San Diego, CA), and mouse anti-vGlut3 (1:25; cat no.: 135211; Synaptic Systems, Goettingen, Germany) at 4 C overnight.

Techniques: Immunolabeling, Labeling

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Regeneration of Cochlear Hair Cells and Hearing Recovery through Hes1 Modulation with siRNA Nanoparticles in Adult Guinea Pigs.

doi: 10.1016/j.ymthe.2018.03.004

Figure Lengend Snippet: Figure 5. Immature IHCs Were Observed in OCs Treated with siHes1 NPs (A–F) HCs were immunolabeled with myosin VIIa antibodies (green in A, B, G, and H), whereas mature IHCs were differentially immunolabeled with vGluT3 (red in C, D, G, and H). Nuclei were labeled with DAPI (blue in E–H). Arrows in (B) and (H) indicate a myosin-VIIa-positive/vGluT3-negative ectopic immature IHC in a noise-injured OC at nine weeks post-siHes1 NP treatment. Arrowheads in (B), (D), and (H) indicate a myosin VIIa/vGluT3-positive ectopic IHC in the same siHes1-NP-treated OC. All IHCs in OCs treated with scRNA NPS were double labeled with myosin VIIa and vGluT3 (G). The scale bar represents 10 mm in (H) for (A)–(H).

Article Snippet: After blocking, the tissues were incubated with rabbit anti-myosin VIIa, goat anti-prestin (1:20; cat no.: OAEB00391; Aviva Systems Biology, San Diego, CA), and mouse anti-vGlut3 (1:25; cat no.: 135211; Synaptic Systems, Goettingen, Germany) at 4 C overnight.

Techniques: Immunolabeling, Labeling